Extracting kākāpō semen for artificial insemination

Image: DOC

Introduction

We research new ideas to improve the size and long-term health of the kākāpō population.

Highlights

We collaborate with local and international experts in research fields such as genetics, artificial insemination and nutrition.

In this section

Artificial insemination

Kākāpō sperm.
Kākāpō sperm
Image: Andrew Digby | DOC

Infertility and inbreeding

Since 1985, 40% of all kākāpō eggs laid have been infertile, and another 20% of embryos have died early in development. It’s a much higher level of infertility than that observed in other parrot species – usually 10–15%. 

We suspect inbreeding is the main cause of high infertility in kākāpō. Most kākāpō from Stewart Island/Rakiura share an unusually high proportion of genes. 

Assessing male fertility

We’ve found some male kākāpō to have a high number of abnormal sperm. This could explain the high incidence of infertile eggs and dead embryos.

To reduce infertility, we’ve removed the poorest performing males from the breeding population.

Simulating multiple mating

We know that females that mate more than once lay significantly more fertile eggs than those that mate once only.

We don't know how to encourage female kākāpō to mate more frequently. But with artificial insemination we can replicate a second mating, hopefully improving fertility.

We produced the first chicks from artificial insemination in the 2009 breeding season.

Promoting genetic diversity

Artificial insemination can minimise the loss of genetic diversity. We use DNA fingerprinting to select sperm donors with rare genes and relatively few offspring. We freeze the sperm for later use using cryopreservation.

We can use DNA fingerprinting to determine whether new chicks resulted from artificial insemination or natural mating.

Experts we collaborate with

  • Dr Juan Blanco (Centro de Estudios de Rapaces Ibéricas, Spain) is an expert in cryopreservation and artificial insemination with non-domestic birds such as eagles and falcons.
  • Dr Serean Adams (Cawthron Institute, New Zealand) has made breakthroughs in the cryopreservation of shellfish sperm.

Genetic studies

Minisatellite DNA analysis has helped us determine the paternity of chicks produced in recent years. Now, micro-satellite primers give us a far greater understanding of the relationships between birds.

Minisatellite and microsatellite DNA sequences show that the Stewart Island/Rakiura-descended kākāpō population has low genetic diversity. This is supported by inbreeding signs including poor egg fertility and hatchability.

The last Fiordland kākāpō, Richard Henry, and his three offspring have the greatest degree of genetic variation. These birds are extremely valuable to help overcome kākāpō inbreeding issues.

Microsatellite DNA analysis helps us avoid kākāpō inbreeding, maximising the productivity and genetic diversity of the next generation of kākāpō.

Kākāpō125+ gene sequencing

Experts we collaborate with

  • Dr Bruce Robertson (Otago University) 

Supplementary feeding trials

Kākāpō feeding at hopper.
A kākāpō feeds on supplementary food at a smart hopper
Image: Andrew Digby | DOC 

More food – more eggs

We've found that female kākāpō lay larger clutches when provided with extra nutritional food. Our supplementary feeding programme ensures that kākāpō are healthy enough to breed.

Too much food – too many males

Overfeeding is counterproductive. If mothers are given too much supplementary food, they tend to produce more sons than daughters.

Our goal is to achieve more breeding females, so we carefully monitor the weights of individual females and adjust their amount of supplementary food accordingly. We can equalise the number of male and female chicks produced this way.

A rimu fruit fallback

Four stages of rimu fruit maturation.
Rimu fruits showing ripening stages
Image: Andrew Digby | DOC 

We’re developing a supplementary food that mimics the nutritional composition of ripe rimu fruit.

Breeding in kākāpō is triggered by abundant rimu fruit crops, which occur every three years on average. Often, the rimu crop fails to ripen, and we must hand rear most or all chicks to prevent their starvation. It’s expensive and labour intensive, and the chicks grow more slowly than they would with sufficient rimu fruit.

A successful new supplementary food would allow female kākāpō to successfully raise their chicks even when the rimu fruit crop fails to ripen.

Experts we collaborate with

  • Yvette Cottam (Massey University, New Zealand)
  • Professor David Raubenheimer (Massey University, New Zealand)
  • Professor Kirk Klasing (University of California, USA)

Other research

'In/out' trials

Feeding known quantities of kahikatea fruit and different hand-rearing formulae to chicks, to determine the quantities of specific nutrients chicks obtain from these foods.

The information will help us to formulate a supplementary food that female kākāpō can raise chicks on.

Faecal analysis

Research to identify any dietary triggers to kākāpō breeding. Studying kākāpō droppings …

Hormone therapy

Research to artificially induce breeding in female kākāpō through the application of female reproductive hormones.

Kākāpō habitat selection

Research to determine the carrying capacity of Codfish Island/Whenua Hou for kākāpō, and identify habitat features that influence kākāpō productivity.

Artificial stimulation of rimu fruiting

Research to determine whether rimu trees can be stimulated to fruit through the artificial application of plant hormones.

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